Flow Cytometry This lab is fully equipped with a state-of-the-art cytometer (described below) and analysis software. This BD Fortessa flow cytometer is equipped with 4 lasers: a 488nm (2-color detection), a 640nm (3-color detection), a 405nm (6-color detection), and a 561nm (4-color detection). It also has high throughput capability (contact: Jason L. Hellmann, PhD).
Analysis of Live Cellular Metabolism The Core also houses an extracellular flux analyzer (Agilent XFe96) to measure metabolism in live cells, small organisms, and tissue explants. In addition, these instruments can be used to measure electron transport chain activity in isolated mitochondria or permeabilized cells. These systems measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), which are indicators of mitochondrial respiration and glycolysis, respectively. These measurements provide insights into metabolic function (contact: Bradford G. Hill, PhD).