Contact: Jason Hellmann, PhD
Inflammatory Lipid Mediators The Metabolic Profiling Core has developed and validated an LC-MS/MS method for the absolute quantification of polyunsaturated fatty acid-derived lipid mediators (including lipoxins, resolvins, maresins, protectins, and pathway intermediates). Samples are processed using the Biotage Extrahera automated extraction system, and data acquisition is conducted on a Sciex 5500 QTrap mass spectrometer coupled with a Shimadzu Prominence HPLC system. Sciex OS-Q software is used for data processing, enhancing consistency, reproducibility, and accurate quantification across samples and studies.
Analysis of Live Cellular Metabolism The Core also houses an extracellular flux analyzer (Agilent XFe96) to measure metabolism in live cells, small organisms, and tissue explants. In addition, these instruments can be used to measure electron transport chain activity in isolated mitochondria or permeabilized cells. These systems measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), which are indicators of mitochondrial respiration and glycolysis, respectively. These measurements provide insights into metabolic function (additional contact: Bradford G. Hill, PhD).
Metabolic/Behavioral Phenotyping System The Center has an 8-cage behavioral and metabolic phenotyping Promethion Core system (Sable Systems International) outfitted with voluntary wheels and programmable access to food and water. The system is equipped with a stable isotope gas analyzer for analysis of nutrient utilization and the system operates within an environmentally controlled cabinet with programmed passcode protected accessibility. Using Promethion Live software, real-time data for each parameter of every cage can be viewed, allowing users to make key decisions at any time, from any place. We also have a complete TSE PhenoMaster/LabMaster Metabolic Chamber system to monitor individual mouse metabolic functions including indirect calorimetry (O2 consumption, CO2production, RER), food intake, water consumption, and total activity (ambulatory and fine motor activity in X-axis).
Flow Cytometry This lab is fully equipped with a state-of-the-art cytometer (described below) and analysis software. This BD Fortessa flow cytometer is equipped with 4 lasers: a 488 nm (2-color detection), a 640 nm (3-color detection), a 405 nm (6-color detection), and a 561 nm (4-color detection). It also has high throughput capability.
Analysis of Live Cellular Metabolism The Core also houses an extracellular flux analyzer (Agilent XFe96) to measure metabolism in live cells, small organisms, and tissue explants. In addition, these instruments can be used to measure electron transport chain activity in isolated mitochondria or permeabilized cells. These systems measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), which are indicators of mitochondrial respiration and glycolysis, respectively. These measurements provide insights into metabolic function (additional contact: Bradford G. Hill, PhD).
Metabolic/Behavioral Phenotyping System The Center has an 8-cage behavioral and metabolic phenotyping Promethion Core system (Sable Systems International) outfitted with voluntary wheels and programmable access to food and water. The system is equipped with a stable isotope gas analyzer for analysis of nutrient utilization and the system operates within an environmentally controlled cabinet with programmed passcode protected accessibility. Using Promethion Live software, real-time data for each parameter of every cage can be viewed, allowing users to make key decisions at any time, from any place. We also have a complete TSE PhenoMaster/LabMaster Metabolic Chamber system to monitor individual mouse metabolic functions including indirect calorimetry (O2 consumption, CO2production, RER), food intake, water consumption, and total activity (ambulatory and fine motor activity in X-axis).
Flow Cytometry This lab is fully equipped with a state-of-the-art cytometer (described below) and analysis software. This BD Fortessa flow cytometer is equipped with 4 lasers: a 488 nm (2-color detection), a 640 nm (3-color detection), a 405 nm (6-color detection), and a 561 nm (4-color detection). It also has high throughput capability.
Exercise Systems The Center has three Columbus Instruments six-lane rodent treadmills with software-controlled capabilities. The Center also maintains 50 in-cage voluntary exercise mouse running wheels equipped with VitalView activity software for 24 h automated recording. In addition to forced and voluntary exercise systems, the Core maintains two 15-gallon tanks for swimming exercise in mice.